BIOTECHNOLOGY SYLLABUS (Science Stream)
Academic Year (2021 - 22)
SYLLABUS FOR HIGHER SECONDARY FINAL YEAR COURSE
One Paper - Time - Three Hours - Marks 100
Unit wise Distribution of Marks and Periods:
|
Unit No. |
Title |
Marks |
Periods |
|
Unit-1 Unit-2 Unit-3 Unit-4 Unit-5 Unit-6 |
Protein Engineering and Bioinformatics. Genetic Engineering and genomics. Environmental Biotechnology & Bioethics. Microbial Technology. Plant Cell Culture Technology. Animal Cell Culture Technology. |
15 15 10 10 10 10 |
23 32 20 25 25 25 |
|
|
Total |
70 |
150 |
Unit-1: Protein Engineering and Bioinformatics: 15
v Protein
based products and designing.
v Proteins.
v Proteomics:
an introduction.
v Introduction
to Bioinformatics.
v Sequences
and Nomenclature.
v Information
Sources.
v Analysis
using Bioformatics tools.
Unit-2: Genetic Engineering and Genomics: 15
v Recombinant
DNA technology-definition and tools.
v Making
recombinant DNA.
v Construction
of DNA library :
v Genomic
and CDNA.
v Cloning
vectors.
v Polymerase
Chain Reaction (PCR).
v DNA
probes.
v Hybridization
techniques: Southern, Northern and Western.
v DNA
sequencing.
v Genomics:
an introduction.
Unit-3: Environmental Biotechnology & Bioethics: 10
v Bioremediation
of oil pollution reducing environmental impact of chemical
v herbicides
& fertilizers; biosensors to detect environmental pollution.
v Biofertilizers-definition
and uses.
v Biofuels
: definition and application
v Genetically
Modified Organisms and
v Ethical
Issue.
v Intellectual
Property Rights-Patenting Life form.
Unit-IV: Microbial Technology 10
v Classification
of microorganism.
v Microbial
culture techniques.
v Measurement
and kinetics of microbial growth.
v Strain
Isolation and Isolation of microbial products.
v Application
of microbial culture.
Unit-V: Plant cell culture Technology 10
v Introduction;
Cellular Toti potency.
v Plant
cell and tissue culture techniques and media.
v Application
of plant tissue culture.
v Gene
transfer methods in plants.
v Transgenic
plants for crop improvement.
Unit-VI: Animal Cell Culture Technology 10
v Introduction.
v Animal
Cell Culture Technology and media.
v Characterization
of cell lines.
v Scale
up of animal cell culture process.
v Application
of animal cell culture.
v Stem
cell technology.
SYLLABUS
FOR BIOTECHNOLOGY PRACTICAL
Total
Marks-30
Scheme of Evolution: Marks
1. Two Experiments 8 + 8 =
16
(One computer based Practical)
v Data
retrieval and data search using Internet site of NCBI.
v Download
a DNA protein sequence from Internet, analyze and comment over it.
v Ion-exchange
chromatography for protein.
v Estimation
of DNA.
v Isolation
of microbes from a given biological sample.
v Sterilization
techniques: Dry heat and moist heat sterilization, Chemical sterilization and
ultra filtration.
v Determination
of bacterial growth curve.
v Determination
of blood groups.
v Estimation
of blood glucose by enzymatic method.
v Demonstration
of plant tissue culture technique.
v Isolation
of bacterial plasmid DNA and its detection by gel electrophoresis.
v Minor
project work/Seminar
2. Viva on practical 4
3. Practical Record 4
4. Seminar/Minor project 6
Recommended Books
1. CBSE publication for class XI and XII
ANNEXURE
Laboratory Requirements:
A. Must include the following components for Laboratory:
a)
One small lab with Laminar Air flow cabinet and
Single working desk
b)
Working Laboratory with working table & Chairs,
Washing facilities, light arrangement.
c) Essential equipments: Autoclave, oven,
Refrigerators, Incubator, Water distillation, Centrifuge.
d)
Glassware’s, measuring equipment etc.
e) Small Culture room (air conditioned) and culture racks.
***
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